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How do the NE-PER Reagents fractionate cellular proteins?

cellular fractionate ne-per Proteins reagents
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How do the NE-PER Reagents fractionate cellular proteins?

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The CER I Reagent induces swelling of the cell causing stress on the cellular membrane. The CER II reagent gently lyses the cell membrane allowing cytoplasmic proteins to be collected, while leaving the nucleus intact. The NER Reagent is then used to extract nuclear proteins from the pellet.

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