How Do You Use Diacetyl Monoxime?
Diacetyl monoxime, chemically known as CH3C(=NOH)COCH3, is a white to off-white powder that’s slightly soluble in water. It’s used as a reagent in a number of reactions in organic chemistry, especially for the detection of urea. The Blood Urea Nitrogen (BUN) test is used primarily to detect problems with the kidneys and uses diacetyl monoxime as its primary reagent. This test is routinely performed in many medical laboratories. Label the three test tubes as “Blank,” “Test” and “Standard.” Fill the “Blank” test tube with 0.05 ml of distilled water, the “Test” test tube with 0.05 ml of the blood serum sample and the “Standard” test tube with 0.05 ml of a standard solution. The standard solution represents a reference BUN concentration, usually about 15 mg of urea per deciliter of blood (mg/dl). Add 3.0 ml of diacetyl monoxime to all three test tubes and mix it thoroughly by lateral tapping (tapping on the side of the test tube). Add 3 ml of ferrithiocyanate to each test tube and mix thor
