How does an ELISPOT assay differ from an ELISA?

assay differ ELISA elispot
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How does an ELISPOT assay differ from an ELISA?

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An ELISA determines the total concentration of the secreted cytokine, whereas an ELISPOT enumerates cytokine secreting cells answering the question ‘what is the frequency of secreting cells?’. Therefore ELISPOT should be used not ‘instead of ‘ but rather ‘in addition to’ ELISA. An ELISPOT assay can be up to 400 times more sensitive than a conventional ELISA because the cytokine is captured directly onto a plate before having the change to be diluted in the culture supernatant, degraded by proteases or captured by cytokine receptors on adjacent cells.

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