How does Clearview TB ELISA detect LAM antigen?
Antibodies adsorbed on the ELISA plate capture the carbohydrate surface antigen found in positive test samples. The conjugated antibodies then attach to the captured antigen creating a sandwich assay. In the presence of the color developer, a color change occurs. The assay reaction is stopped using the stop solution, and the intensity of the color (optical density) is measured using a microtiter plate reader. A positive result indicates that LAM antigen of mycobacteria is present in the sample, whereas a negative result indicates that it is not present at or above the test’s detection limits.
