How does JPT Peptide Technologies QC the synthesis of membrane-bound peptides?
In addition to the membrane bound PepSpot peptides ordered, JPT Peptide Technologies assembles control peptides on the same membrane, which are connected via a cleavable linker system to the cellulose surface. After cleavage of these peptides from the membrane, their quality will be checked by MALDI-MS. The control peptides include specific sequences which are difficult to synthesize. The purity of the peptides synthesized varies for each peptide and is dependent on the sequence and length. Our analyses have determined that peptide purity is typically >70% for average 6-15 mers (by HPLC and mass spectroscopy).
