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How does PRIMERFOX calculate the melting/annealing temperature?

April 26, 2017annealing CALCULATE melting primerfox temperature

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How does PRIMERFOX calculate the melting/annealing temperature?

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PRIMERFOX calculates the melting temperature (Tm) very simple but sufficient for most PCR applications. The calculation is: Temp = 4(G + C) + 2(A + T) C One should aim at using an annealing temperature about 2 to 5C below the lowest Tm of the pair of primers to be used (Innis and Gelfand, 1990).