How does Sigma-Genosys QC the synthesis of SPOTs peptides in the custom SPOTs service?
Sigma-Genosys synthesizes three standard control peptides at the end of every custom SPOTs project, of which 2 positive and negative control peptides are probed with a standard monoclonal antibody. A third peptide is synthesized with a cleavable linker that enables cleavage of the peptide from the membrane for analysis with mass spectroscopy for mass determination and HPLC for purity.
