How does the paternity test work?
Every individual person has their own unique DNA fingerprint, which includes markers that come from each parent. These markers are called microsatellites. VITA-TECH’s state-of-the-art technology compares the microsatellite variations of a child to those of the presumed parents. This technology can conclusively verify the biological parents of each individual. VITA-TECHs DNA paternity test utilizes thirteen different microsatellite markers to reduce the possibility of error to virtually zero. Example: The diagram below demonstrates how DNA paternity testing works. For simplicity, the data is depicted for only one microsatellite marker. Polymerase Chain Reaction (PCR) amplifies the targeted DNA microsatellite and the DNA is then separated by size. Larger pieces are at the top, and smaller pieces are at the bottom. Each person has two copies of the marker, so there are two PCR products. M = Mother, C1-2 = Two Children, F1-2 = Two Alleged Fathers The mothers (M) two copies of the microsate
• When will I receive the paternity test results? • What is the meaning of a court-admissible paternity test? • What collection methods for my paternity test are available? • How much is a paternity test? • How accurate is a paternity test? • Who needs to be involved in a paternity test? • How can I get my paternity test results? • Are my paternity test results confidential? • How do you ensure that paternity tests are performed correctly? • Will the paternity test results give a conclusion that is 100% certain? • What is the difference between an AABB (American Association of Blood Banks) accredited laboratory tests and a non-accredited laboratory tests? • What does it mean for a drug sample to have FDA (Federal Drug Administration) approval? • Is there an age limit for the DNA test? • Do DNA test samples have to be collected at the same time? • How does the Home Test Kit work? • What is a buccal swab collection, what is blood collection? • How will I receive my laboratory test result
DNA is taken from the sample or blood, cheek cells, or cultured cells. Enzymes are used to cut the DNA sample in fragments which are placed into a gel matrix. An electric current is used to drive the fragments across the gel, making the smallest fragments move the farthest and the larger fragments move the shortest distance. The separated DNA fragments are transferred to a nylon membrane. This membrane is exposed to a labeled DNA probe (a short piece of customized DNA that recognizes and binds to a unique segment of the tested person’s DNA). The nylon membrane is then placed against a film which, after it is developed, reveals black bands where the probes are bound to the DNA. The child’s visible band pattern is unique, with half matching the mother and half matching the father. The process is repeated several times with different probes, each identifying a different DNA area and producing a distinct pattern. Using several probes creates a greater than 99.9% certainty about paternity o
A child inherits half of their DNA from the biological Mother and half their DNA from the biological Father. The DNA profiles are then compared (on a 16 genetic marker basis) to establish whether the alleged father is indeed the biological Father of the child. If the alleged father is the biological Father of the child, then all the loci will match and he will be included as being the Father with a guaranteed probability in excess of 99.99% when the mother is included. The probability is normally over 99.9% if the Mother is not included. If the man tested is not the biological Father of the child he will be 100% excluded resulting in a probability of paternity of 0%.