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How I do prepare powdered silica for DNA purification, with the associated solutions?

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How I do prepare powdered silica for DNA purification, with the associated solutions?

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• Sigma Chemical Co. (St. Louis, MO). “Silica”; do not used “fumed silica”. • Cutter Ceramics (11908 Old Baltimore Pike, Beltsville, MD 20705). Ask for 325 mesh powdered flint glass fines. Resuspend 400 g of silica powder in 800 ml ddH2O in a 2 liter flask. Stir for 60 mins. Allow to settle for 90 mins. Take the SUPERNATANT (which contains the “fines” of interest) and pellet in Sorvall (GSA rotor, 10 mins at 6000 rpm). Resuspend pellet in 200-300 ml ddH2O. Add nitric acid to 50 %. Bring close to boiling in fume hood. Allow to cool. Pellet silica as before, wash pellet 4-6 times with ddH2O (check that the pH returns to neutral). Store final pellet as 50 % slurry in ddH2O. Store at -70 deg C, working aliquot at 4 deg C. Recipe #2: Do a size (1-g sedimentation) cut, taking the powder that settles out between 2 mins and 60 mins; then boil it all in 10 volumes of 50% nitric acid for an hour, and wash extensively. PREPARATION OF BINDING AND WASH SOLUTIONS: NaI (BINDING) SOLUTION: Recipe #1:

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