How is it possible to check which osmolarity of electroporation buffer is still tolerable for a cell line?
For this purpose, the cells are incubated in different mix ratios of hypoosmolar and isoosmolar buffer for 30 min. Starting from the hypoosmolar buffer, osmolarity should be increased in increments of approx. 50 mOsmol. The survival rate of the cells is then examined (e.g. using Trypan blue staining). Taking account of the fact that at least 90 % of the cells in the buffer must survive, the buffer with the lowest possible osmolarity should be selected. A mixing table is included in the Basic Application Manual for electroporation, for example.
