How is NimbleGens technology different from other in situ technologies?
Other commercially available in situ synthesis technologies suffer from either low feature density or poor design flexibility making them less attractive for custom DNA array synthesis. The predominant method is based on the photochemical synthesis of high quality arrays, however the method relies on the use of chromium lithographic masks, rather than an addressable mirror array, to generate the desired light patterns for array synthesis. Since the preparation of physical masks is an expensive process, the correction of error in array designs and the preparation of custom arrays are costly. Other methods rely on the sequential deposition of phosphoramidites or deprotection reagents within the growing array to control synthesis in a site specific manner. While these methods have some of the flexibility advantages of NimbleGen arrays, they are ultimately limited by the print resolution of their deposition process. As a result the total number of probes available is typically 10 fold lowe