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How is RNAse contamination avoided?

avoided contamination rnase
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How is RNAse contamination avoided?

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The incubation columns must be sterilized prior to a run. There is also an automated cleaning procedure available for the dispensing needle and tubing. Thorough rinsing procedures are programmed to avoid RNAse contamination even if RNAse digestion has been used as a step in the protocol. The solvent used to rinse the needle is separate from reagents delivered and can be DEPC treated for added protection.

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