Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

How many qPCR reactions will I get from one WT-Ovation™ FFPE amplification?

April 26, 2017amplification ffpe qPCR reactions wt-ovation™

0

Posted

How many qPCR reactions will I get from one WT-Ovation™ FFPE amplification?

1 Answer

0

Posted

The number of qPCR reactions depends on the abundance level of the genes being interrogated. For medium to high copy genes, the cDNA may be diluted as much as 400-fold, enough for thousands of qPCR reactions. For very low copy genes more cDNA must be used per qPCR reaction. We recommend purification of the amplified cDNA prior to qPCR analysis.