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How many qPCR reactions will I get from one WT-Ovation™ Pico amplification?

April 26, 2017amplification Pico qPCR reactions wt-ovation™

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How many qPCR reactions will I get from one WT-Ovation™ Pico amplification?

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The number of qPCR reactions depends on the abundance level of the genes being interrogated. For medium to high copy genes, the cDNA may be diluted as much as 400-fold, enough for hundreds of qPCR reactions. For very low copy genes you will need to use more cDNA per reaction. The user will need to determine how much cDNA to use per reaction depending on the abundance of the gene being interrogated. Note that we recommend purification of the amplified cDNA prior to qPCR analysis. Note that we recommend purification of the amplified cDNA prior to qPCR analysis.