How much antigen is required for immunization?
Depends upon the immunogenity of the antigen. In the standard protocol (for rabbit, goat or hen) we use: • 200 g of protein or peptide for the first immunization • 100-200 g of protein or peptide for the following one In total: • around 800 g of peptide/animal/14 weeks program • around 500 g of protein/animal/whole 14 weeks program Lower amounts of antigen (less than 10 g) are acceptable in cases of low relatedness between the antigen and proteins of the animal chosen for immunization. We can also use your own immunization protocol. If protein needs to be concentrated, please be aware, that in some cases it can be lost by attaching to the membrane used in a concentrating device.
