How should I decide between a PCR or QPCR genotyping assay?
PCR assays can determine zygosity of knockouts, knock-ins, and point mutations (with subsequent restriction digest). They can also determine carrier status (but not zygosity) of transgenes. QPCR assays can determine zygosity (hemizygote versus homozygote) of a transgene. The assay can also be used to determine trangene copy number.