How should I reconstitute the VERIFY tagged antigen standard for antibody validation?
For Western Blot validation, the overexpression lysate sample can be diluted with 2xSDS sample buffer. After dilution, the lysate sample should be stored at -80℃ for long term storage. Prior to SDS-PAGE fractionation, add reducing agent (100mM DTT or 2.5% ß-ME) freshly and heat the sample at 70℃ for 10 minutes before loading.
