How should spectral resolution be defined?
Simple, quote the FWHM at the most likely slit width, and show an example of a spectrum of an ion lamp feature, such as the Hg+ 436 nm line, or the 405 – 408 nm doublet. Meticulous bio-scientists, physicists, and chemists demand proof of performance; a spectrum is both an unambiguous way to define the system, and also a good way to compare instruments. Click here for a comparison between prism and diffraction grating performance. Back to top • Fast characterization of extended samples: Fast scan of up to 50 mm long samples, automatically, in a single, unbroken sequence, at any microscope objective magnification, from 2 to 100X. Ideal for smears and biopsy samples. Back to top • Spectral “Unmixing”: Unlike competitors PARISS algorithms do not assume linear spectral mixing! Click here for the LightForm approach and an unmixing “rant”. Back to top • Extreme sensitivity: As previously noted, researchers have performed live-cell FRET using PARISS – with a video camera! You can now read two
