How to determine DNA Sequencing Data Quality?
At 1st BASE, the trace data quality is measured by a series of statistical measurements. These statistical measurements were developed by genome sequencing centres to ascertain DNA sequencing data quality. These are not standards set by 1st BASE but by these very same genome centres. The statistical measurements are embedded into our trace files and can be read by certain trace viewers. Quality Values (QV) The Applied Biosystem 3730 Genetic Analyzer uses Applied Biosystems DNA Sequencing Analysis Software v5.2 with KB Basecaller. The KB basecaller assigns quality values (QV) to each basecall (pure and mixed bases). The QV is calculated using the following equation: QV = -10log10 (PE) where PE is the probability that a basecall is erroneous. The calculated QV for each base may be visualised on the analysed data as coloured QV bars above each basecall. These QV bars are only visible using the DNA Sequencing Analysis Software or Applied Biosystems SequenceScanner program. The software def
