How to homogenize kidney tissue?
Materials and methods Tissue samples were taken from various species and organs, as indicated in the figure legends. For stabilization using RNAlater TissueProtect Tubes and RNAlater RNA Stabilization Reagent, samples of the appropriate size were immediately placed in the reagent. Disruption and homogenization on the TissueLyser was carried out according to the TissueLyser Handbook. Rotor–stator homogenization was carried out on individual samples for 1 minute each. Total RNA was purified using the RNeasy Mini Kit, RNeasy Lipid Tissue Mini Kit, RNeasy Fibrous Tissue Mini Kit, or RNeasy Fibrous Tissue Midi Kit, as indicated. Total RNA yields were determined by absorbance at 260 nm, and total RNA was analyzed by formaldehyde agarose gel electrophoresis. Results and discussion In order to compare the TissueLyser with standard rotor–stator homogenization, various rat tissues were processed, and total RNA was purified using RNeasy Kits. Disruption and homogenization using the TissueLyser ga