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I am preparing my own biotin solutions. Why am I having problems with the biotinylation reaction?

Biotin biotinylation preparing problems REACTION solutions
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I am preparing my own biotin solutions. Why am I having problems with the biotinylation reaction?

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We have noticed that the biotin from some suppliers tends to ‘go off’ rapidly. We routinely use biotin from Research Organics, as this is the most stable we have come across.

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