I have cultured mouse ES cells in my lab. Should I expect human ES cells to behave and look similar to mouse ES cells?
No, human ES cells will not grow as robustly or in the same manner as mouse ES cells. Your human ES cells will be slower to recover and will generally need more attention than mouse ES cells. 10. I have cultured primate ES cells in my lab, and the components/feeder/media I used were different from what you recommend for human ES cells. Why aren’t the conditions identical, given the similarty of non-human to human primates? Although non-human primate and human ES cells are quite similar, we have observed significant differences in their responses to different media. Use the provided protocols for your hES cells for best results.
