I have viewed my cells in the CELLine classic but when I tried to view them again after several days of culture I was not able to focus on them, what has changed?
When the CELLine is removed from the incubator and the temperature of air in the CELLine is cooled slightly, contraction of the air takes place and will draw the membranes of the cell compartment up into the device. This contraction lifts the bottom membrane and takes it out of focusing distance. Momentarily loosening the medium compartment cap will equilibrate pressure and return the membrane to its original position.
Related Questions
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