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I need to use trypsin to treat the protein before doing my experiment, since you have protease inhibitor cocktail, I wonder if those inhibitors will inhibit the activity of trypsin?

April 26, 2017cocktail doing experiment inhibitor Inhibitors protease protein treat trypsin wonder

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Posted

I need to use trypsin to treat the protein before doing my experiment, since you have protease inhibitor cocktail, I wonder if those inhibitors will inhibit the activity of trypsin?

1 Answer

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Yes, you need to get rid of the protease inhibitors before doing your experiment in such case.