I want to collect my tissue culture supernatant daily for a time-course study and run all of my samples at once by ELISA. How should I store my supernatants?

collect culture ELISA run samples study supernatant time-course tissue
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I want to collect my tissue culture supernatant daily for a time-course study and run all of my samples at once by ELISA. How should I store my supernatants?

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The supernatants can be harvested and stored -20°C. Avoid freeze/thaw cycles. It is recommended to briefly centrifuge samples after collection to pellet any dead/floating cells remaining in the supernatant. Transfer to new tube for storage.

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