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I want to cultivate Escherichia coli in a microtitre plate. Which conditions should I choose?

April 26, 2017coli Conditions cultivate ESCHERICHIA microtitre plate

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I want to cultivate Escherichia coli in a microtitre plate. Which conditions should I choose?

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You should use 96-deepwell plates equipped with air-permeable sealing tape as a sterile barrier. The filling volume should not be greater than 500µL. Shake the plates with an orbital speed of 300 rpm and a shaking diameter of 50 mm. With smaller shaking diameters you should increase the shaking speed. For further information consider the following publication: Characterization of Gas-Liquid Mass Transfer Phenomena in Microtiter Plates Hermann R., Lehmann M., Buechs, J. Biotechnology and Bioengineering (2003); Vol. 81, No.