I work in an automation lab and want to use the cocktails on a liquid handling workstation. Are Calbiochem® protease inhibitor cocktails stable?
Our cocktails are compatible with use on liquid handlers and are stable as shown in the data below. Stability of Protease Inhibitor Dilutions in BugBuster® Protease inhibitors were diluted to the prescribed working concentration (Competitor; Calbiochem® Cocktail VII, Cat. No. 539138). The ability of the inhibitors to inhibit the proteolytic activity of PRONASE® (Cat. No. 537088) was measured by using the Universal HT Protease Assay on days zero, one (24 h post dilution), three (72 h) and five (120 h). The Universal HT Protease Assay quantifies protease activity using a fluorescein thiocarbamoyl-casein derivative (FTC-casein). Proteolytic activity liberates FTC-labeled peptides, which results in enhanced fluorescence (Ex.max: 495 nm; Em.max: 525 nm). Addition of the protease inhibitor cocktails inhibits the proteolytic activity of the PRONASE® (Cat. No. 537088), resulting in reduced fluorescence. On day 1, for samples incubated at 8°C, the competitor tablet inhibited the proteolytic act