If my antibody is very dilute (e.g. 5 gg/ml) and I have 5 ml of solution, which PhyTip column do you recommend and how do I recover as much protein as possible?
I ujd A: Using the 1000+ PhyTip columns, divide the 5 mL sample into 5 equal volumes of 1 mL. With the same PhyTip column, perform the Capture step at least 2 times for each 1 ml of sample sequentially. Enrich the total protein captured in the one PhyTip column in 15 gl of elution buffer. Alternatively, the sample could be put into at least a 6 mL vial and the 1000+ PhyTip column could be used to capture the entire sample provided enough capture cycles are used. In this case, approximately 15 cycles should be used to capture the entire sample. After sample capture, process the PhyTip column in the normal manner.