Im interested in post-translational modifications in my protein. Can I get complete sequence coverage of my protein by mass spectrometry?

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Im interested in post-translational modifications in my protein. Can I get complete sequence coverage of my protein by mass spectrometry?

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It is difficult to get complete coverage of any protein. It depends on the abundance, purity, protein sequence, hydrophobicity, modifications to the protein that may inhibit digestion (such as glycosylations), etc. Analyzing the protein using different enzymes and using multiple strategies of analysis will increase the coverage.

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