In what formats can the shRNA clones be ordered?
Sigma-Aldrich is both a collaborative member and supplier of TRC1 and TRC2. The TRC1 library currently contains more than 159,000 pre-cloned shRNA constructs targeting 16,000 annotated human genes and 15,950 annotated mouse genes. The TRC2 library contains more than 150,000 pre-cloned shRNA constructs. Most genes have at least 3 individual clones from which to choose. Three formats are available: bacterial glycerol stock, purified plasmid DNA, and lentiviral particles. For more information on ordering MISSION® products, please click here. If I want to make my own viral particles, which format should I purchase? Sigma offers ready-to-use viral particles to eliminate these steps for you. However, if you choose to make your own viral particles, we recommend the purified plasmid format for packaging protocols that require less than 1 µg of plasmid DNA (i.e. typical protocols for 60 mm dishes utilize 1 µg of transfer vector and yield 4-5ml of viral particles). For large scale production of
