Is a special sample preparation protocol required for mouse cells?
Generally, a special sample preparation protocol for the isolation of mouse cells with MACS® Technology is not required. Exceptions are the isolation of dendritic cells and liver sinusoidal endothelial cells (LSECs) where collagenase treatment is strongly recommended to increase the recovery of the cells. For the isolation of LSECs from liver, a density gradient centrifugation has to be performed. A single-cell suspension is the prerequisite for optimal separation results. Cell aggregates may decrease the purity of the positive fraction as they may contain both target cells and non-desired cells. In order to remove cell aggregates, the cells should be passed over a Pre-Separation Filter before loading them onto a cell separation column.
