Is it necessary to calculate the quantitative perfusion image series for data analysis?
It helps, but in some cases is not strictly necessary. Because the procedure to generate quantitative perfusion images generally involves calibration using a reference image, absolute perfusion series are less sensitive to variations in the scanner condition. This is important for comparison of temporally widely-spaced blocks. Perfusion quantification will also reduce the inter-subject variability in the group analysis as cerebral blood flow tends to be quite consistent in healthy adult subjects. If absolute perfusion quantification is not possible, the subtracted (relative) perfusion image series can be used for most paradigms except those involving scans on different days.
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