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Is non-specific DNA added to the blocking buffer or hybridization buffers?

April 26, 2017added blocking buffer Buffers dna hybridization non-specific

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Is non-specific DNA added to the blocking buffer or hybridization buffers?

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Yes. The hybridization buffers for the North2South Kit contain single stranded nucleic acids used to block the membrane during the pre-hybridization and hybridization procedures. The addition of more single stranded DNA or RNA is not needed.