Is the library cloning vector available?
The vector used for constructing all three Ph.D. libraries, M13KE, is available for sale. A derivative of M13mp19, M13KE has restriction sites engineered at the 5´ end of gene III permitting construction of custom peptide libraries by insertion of a user-designed synthetic cassette. Because M13KE is a phage, rather than a phagemid vector, all 5 copies of pIII in the processed virions will carry the displayed peptide sequence. Since displayed peptides longer than 20-30 residues have a deleterious effect on phage infectivity, this vector is suitable only for display of short peptide libraries, rather than larger protein or cDNA libraries. The sequence of the vector is available here. The vector is available, for research use only, as part of the Ph.D. Peptide Library Cloning System, product #E8101S. In addition to the vector, this product includes an extension primer for second strand synthesis of the randomized library insert and a detailed protocol for construction of random peptide li
