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Is the LIM-domain protein HaWLIM1 associated with cortical microtubules in sunflower protoplasts?

April 26, 2017associated cortical microtubules protein protoplasts Sunflower

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Is the LIM-domain protein HaWLIM1 associated with cortical microtubules in sunflower protoplasts?

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Brire C; Bordel AC; Barthou H; Jauneau A; Steinmetz A; Alibert G; Petitprez M Laboratoire Biotechnologie et Amélioration des Plantes, INP-ENSAT, BP 107 Auzeville, F-31326 Castanet-tolosan, France. briere@ensat.fr Flowering plants express several LIM-domain proteins related to the animal cystein-rich proteins. The expression of sunflower LIM genes was followed by RT-PCR in cultured sunflower protoplasts. A transcript was detected only for HaWLIM1, but not for the other two genes HaPLIM1 and HaPLIM2. Polyclonal antibodies raised against either full length recombinant HaWLIM1 protein or peptides recognized a 27 kDa polypeptide on Western blots. Immunocytolocalization studies showed that HaWLIM1 is located in the cytoplasm and in the nucleus. In the cytoplasm, HaWLIM1 is localized in punctate structures, distributed along microtubule bundles. Depolymerizing microtubules with oryzalin resulted in a strong modification of the HaWLIM1 cortical pattern. In contrast, treatment of protoplasts wi