Is there a possibility of generating self-replicating viral sequences?
These vectors have been modified to remove sequences that overlap with the packaging plasmid to minimize the possibility for homologous recombination and generation of self-replicating viral sequences when co-transfecting these constructs into packaging cells. The pFIV-H1-Puro and pFIV-H1/U6-Puro vectors also have a deletion in the enhancer of the U3 region of 3-LTR to ensure self-inactivation of the lentiviral vector after transduction and integration of the sequences into the genomic DNA of the target cells.
