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My gene of interest is targeted by multiple shRNA clones, which clones should I assay?

April 26, 2017assay clones gene shrna

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My gene of interest is targeted by multiple shRNA clones, which clones should I assay?

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The Hannon-Elledge shRNA library has, on average, greater than 2 shRNA clones targeting each gene. This redundancy is due to the fact that RNAi-mediated knock-down is variable, and some clones may be less effective than others. Therefore, it is highly recommended that you obtain and assay all available shRNA clones targeting your gene or genes of interest. This relatively minor investment in multiple clones and parallel validation will greatly increase your chances for success. Return to top.