Should I use TaqMan or Sybr Green in the qPCR module of the MeDIP kit?
We have optimized the module with Sybr Green. TaqMan has not been tested in our lab. Nevertheless both can be used as follows: • Sybr Green: following our protocol, using our primers (Sybr Green on DNA sample and internal controls) • If you prefer TaqMan, you should use your own TaqMan primers with your DNA sample, but not with the internal control.
