The application offers three types of standard curve models: point to point, 4P log fit, 5P log fit. Which model is most suitable for my standard curve?

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The application offers three types of standard curve models: point to point, 4P log fit, 5P log fit. Which model is most suitable for my standard curve?

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There is no universal rule for choosing a curve fit, but as a recommendation start with 5PL and work backwards to 4PL and point-to-point until they have a “good fit” as determined by the R squared value. It is also acceptable to delete a true outlier to improve the curve fit.

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