The Coliscan Easygel box indicated it must be frozen for storage, but when I opened the box, the medium was thawed and liquid. Is this a problem?
A. No, the medium can be thawed for 2-3 weeks at room temperature with no adverse effects. Just put the bottles of liquid Coliscan Easygel in the freezer and they will be fine. Also, if you happen to thaw too many to use, they can be re-frozen without any problem. We have kept Coliscan Easygel for a month at room temperatures without noticeable negative effects, but for long term storage life, it is best frozen as the chromogenic substrates slowly lose their brilliant color over time at high temperatures.
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