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The existing BIBAC vectors only have BamHI as a unique site for library construction. Why don you put in some other sites by site-directed mutagenesis, or perhaps a polylinker?

April 26, 2017bamhi bibac Construction existing library site sites vectors

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The existing BIBAC vectors only have BamHI as a unique site for library construction. Why don you put in some other sites by site-directed mutagenesis, or perhaps a polylinker?

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The BIBAC vectors are big. It was difficult to maintain the unique BamHI site. The BamHI site is also compatible is BglII and Sau3A. Other sites that are unique to the BIBAC backbone pCH20 are SrfI, SwaI, PacI and AscI. One (newly available) enzyme that does not cut the existing BIBAC vectors is ApaLI. You can assume that all other commercially available enzymes (6/98) cut the BIBAC vectors at least once.