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The kit insert mentions that single laser flow cytometers can be used but are not recommended due to possible interference of the fluorescence emission from the beads. How?

April 26, 2017cytometers flow insert kit laser mentions possible recommended Used

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The kit insert mentions that single laser flow cytometers can be used but are not recommended due to possible interference of the fluorescence emission from the beads. How?

1 Answer

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A 488 nm laser is capable of exciting both the detector and bead dye fluorophores, however the signals may bleed over from the detector fluorophore which can negatively affect results.