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The volume of supernatant at Step 6 is greater than 800 µL. Do I scale Reagent A?

April 26, 2017greater Reagent scale step supernatant volume

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The volume of supernatant at Step 6 is greater than 800 µL. Do I scale Reagent A?

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There is no need to scale Reagent A unless the volume is greater than 1 mL of supernatant. For each mL greater than 1 mL an additional 25 µL of Reagent A can be added.