We use “supervirulent” Agrobacterium strain EHA101 (or EHA105) for plant transformation. Can we use a BIBAC vector in these strains?
Any A. tumefaciens strain is fine as long as it is not (already) resistant to kanamycin – since BIBAC plasmids carry the gene for resistance to kanamycin as the selectable marker. EHA101 contains a disarmed pTi that is carries resistance to kanamycin thus, is not suitable for use in conjunction with the BIBAC vectors. Also, our experience was that recA- strains performed better for high molecular weight DNA transformation – although this may not always be the case.
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