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What advantage does your GermLineTM mESC (F1) have over the common mES cell lines derived from 129 strains?

April 26, 2017advantage CELL common derived F1 MES mesc

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What advantage does your GermLineTM mESC (F1) have over the common mES cell lines derived from 129 strains?

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Our GermLineTM mESC (F1) line is derived from embryos of 129 svj and C57BL/6. This cell line has been tested to be germline transmitted by tetraploid complementation. Using our GermLineTM mESC (F1), one can generate 100% ES cell derived mice from microinjection (or aggregation) of ES cells into tetraploid blastocysts. No further breeding is necessary. This saves an average of 4 to 5 month time in generating knockout/knockin mice. Our GermLineTM mESC (F1) works for DNA constructs that are either C57BL6 or 129 strain based.