What amount of antibody anty-5-methyl cytidine should be used on Nimblegen platform for MeDIP studies?
The quantity of antibody used for MeDIP is important parameter as using much will increase the background. In our MeDIP kit protocol we recommend to use 0,3 µg per IP (for 1 µg of DNA). It yields in 20-50 ng of recovered DNA. Usually, 1-2 µg of DNA are needed for microarray meaning that immunoprecipitated DNA should be amplified (or if enough of starting material, samples can be pooled).
