What are example absorbance and fluorescence values for the oxidized and reduced forms of alamarBlue®?
Example absorbance values are given in Table 3a and example fluorescent values are give in Table 3b. Since these readings can vary depending on the type of media used, a range of powered media was used. Powdered media was obtained from Sigma and prepared according to their instructions. All media contained phenol red. pH was adjusted to 7.4 with 1N HCI or 1N NaOH. alamarBlue® was added to each media which was then split into 2 samples. One sample of each media was autoclaved for 15 minutes to produce the reduced form. The media were dispensed into a flat bottom Dynatech plate (100μl/well) Table 3a: Absorbance values for oxidized/reduced forms of alamarBlue® for commonly used culture media at different wavelengths. Table 3b: Fluorescence values for oxidized/reduced forms of alamarBlue® for commonly used culture media. Measurements were taken on a Cambridge Technology, Inc. (Watertown, MA) Model 7620 Microplate. Fluorometer-settings were: bottom reading, light source setting 12, no max A
