What are the advantages of the miScript PCR System compared to alternative methods?
A principal advantage of the miScript PCR System is the fact that cDNA made from RNA in a single reverse-transcription reaction can be used to detect multiple miRNAs, other small noncoding RNAs (e.g., snoRNAs, snRNAs), and mRNAs. For example: 100 ng total RNA → 1 RT reaction → 100 PCRs (20 µl reaction volume) Another advantage is the up-to-date design of miScript Primer Assays. Design of miScript Primer Assays is updated every time miRBase is updated. This ensures that you are always working with the most accurate and current miRNA sequence information possible and increases the reliability and accuracy of your experiments. A further benefit is that miScript Primer Assay design is optimized to account for 3′-end polymorphisms. Polymorphisms sometimes occur in the last 2 bases at the 3′ end of an miRNA. This is taken into consideration during the miScript Primer Assay design process. Therefore, you can be confident that all miRNAs are detected even if polymorphisms exist.