What are the critical factors in designing the siRNA molecules to be used for RNAi studies?

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What are the critical factors in designing the siRNA molecules to be used for RNAi studies?

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The algorithm utilized to design the siRNA of interest must assure both exquisite gene-specificity (to minimize off-target effects) and potent efficacy (expression knock down). A minimum of two siRNA molecules must be used for each gene of interest, in order to confirm the gene specificity of any observed changes.

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