What are the reasons for High Intra Assay Coefficient of Variation (CV) (data variation)?
Coating removed by scratching during washing/pipetting: Take care not to scratch the inner surface of the microwells when washing the plate or pipetting standards and samples. Cross contamination from well to well by peeling off plate cover: Carefully peel off the plate cover after incubation to avoid cross contamination from one well to another. Non-homogeneous samples: Make sure the samples you use for the test are homogeneous. Mix them before addition to the plate to avoid any gradients caused by freeze/thawing. Edge effect: Seal the plate during incubation and incubate on a shaker if possible. Pipettes used are not properly calibrated: Make sure the pipettes you use are properly calibrated.
